Abstract:The influence of earthworm and elevated CO2 on cotton leaf decomposition and C and N transformation in the soil by the 56 days experiment treated with earthworm under different levels of CO2 were analyzed. The effect of different levels of CO2, cotton leaf and their interactions on C content in soil was not significant, while interaction of earthworm and cotton leaf was significant. Earthworm didn′t influence the N content solely, but the combined effect of elevated CO2 and earthworm could influence the N content. The interaction of CO2, earthworm and leaf was more significant than that of earthworm and leaf. Our results indicated that the elevated CO2 made the component of litter decomposition easier and improved the ability of decomposition and transformation of litter through earthworm, resulted in the change of C and N content in soil.