Abstract:In order to investigate the impact of Uranium contamination on soil microbial community, the soils were contaminated with different uranium concentrations (i.e. 50, 100, 150 mg·L-1), and the uncontaminated soil were compared as CK. The BIOLOG-ECO technology was used to explore the functional diversity changes and the utilization kinetics of carbon source of soil microbial community. The microbial metabolic activity was represented by average well color development (AWCD) of ECO plate. The results showed that the uranium treatment had an evident inhibition effect on the physiological activity of soil microorganisms. Soil microbial diversity was described with Shannon index, Simpson index and McIntosh index. Results revealed that functional diversity of microbial community in soil treated with uranium was significantly inhibited compared with CK. The ability to use various types of carbon source was different among microbial community in soil. Amino acids and organic amines were mainly utilized by the indigenous microorganisms. The relative utilization rate of 6 carbon sources was lower in uranium treatment groups than CK, in which the utilization of carboxylic acids and polymer compounds declined down to 50%. From principal component analysis, the specific carbon source used by soil microbes in uranium contaminated soil mainly include β-Methyl-D-Glucoside, D-Galactonic Acid Lactone, L-Arginine and so on. In conclusion, uranium treatment can change the soil microbial community structure and lead to the variation of functional characteristics of microorganism physiological metabolism. The method of BIOLOG-ECO technology combined with mathematical statistics analysis is intuitive and quick to reflect soil microbial biodiversity. This research provides a theoretical basis for the assessment and remediation of the uranium contaminated environment.