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作 者 ：黄鹤, 李卓夫, 王晓楠, 付连双, 王姝彦

期 刊 ：核农学报 2016年 30卷 7期 页码：1255-1262

关键词：冬小麦；低温；海藻糖；TaTPS；qRT-PCR；

Keywords:winter wheat, low temperature, trehalose, TaTPS, qRT-PCR,

摘 要 ：海藻糖-6-磷酸合成酶基因(trehalose-6-phosphate synthase, TPS)是海藻糖合成途径中的关键酶基因,在植物耐寒过程中发挥着重要作用。为了分析不同耐寒性的小麦品种中TPS基因在低温处理下的表达差异,明确TPS基因在抗寒中所起的关键作用,以抗寒品种东农冬麦1号(D1)和对照品种中国春(CS)为材料,设定一系列低温处理,用qRT-PCR分析7个TPS基因的表达差异。结果表明,低温驯化初期,TPS4和TPS7在D1中大量表达。低温驯化后期,D1中TPS1、TPS2、TPS4、TPS6和TPS7基因的表达量均明显高于CS中的表达量。TPS2、TPS3、TPS5和TPS6基因在小麦低温冷冻中后期相对表达量达到最高。-16℃下D1中的TPS1、TPS6和TPS7基因相对表达量分别为同时期CS的72.8倍、308.68倍和32.83倍。D1中的TPS3基因在7个冷处理中有6个为下调表达,而TPS7基因在各处理下均为上调表达。本研究结果对高抗寒性新品种的选育具有重要意义。

Abstract:Trehalose-6-phosphate synthase gene (trehalose-6-phosphate synthase, TPS) is the key gene in trehalose synthesis pathway, which plays an important role in the freezing tolerance of plant. To analyze the differences in the expression of TPS between wheat cultivars and determine the key effect of TPS gene on cold tolerance of wheat, expression differences of 7 TPS genes under a series of low temperature treatments in qRT-PCR were analyzed between cod hardy variety Dongnongdongmai1(D1) and cold-sensitive variety Chinese Spring (CS) in this experiment. The results showed that TPS4 and TPS7 were higher expressed in D1 at the beginning of the cold acclimation. The expression of TPS1, TPS2, TPS4, TPS6 and TPS7 in D1 was significantly higher than that in CS in the late period of cold acclimation. TPS2, TPS3 TPS5 and TPS 6 were reached the highest relative expression in the middle and later periods of the cold acclimation. The relative expression of TPS1, TPS6, TPS7 were higher in D1 than in CS under the treatment of-16℃, which were 72.8, 308.68, 32.83 times, respectively. In D1, the expression ofTPS3 was down regulated in 6 of 7 cold treatments, and TPS7 was up-regulation in all treatments. The result of the study had improtant significance to the breeding of new varieties of high cold resistanc.

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