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作 者 ：于威, 颉建明, 滕汉玮

期 刊 ：核农学报 2016年 30卷 8期 页码：1633-1638

关键词：谷胱甘肽；嫁接黄瓜；自毒；种子萌发；缓解效应；

Keywords:glutathione, grafted cucumber, autotoxicity, seed germination, alleviation,

摘 要 ：为探讨外源谷胱甘肽(GSH)对自毒作用的缓解效果和生理作用机制,以连作嫁接黄瓜根际土壤浸提液模拟自毒胁迫,在100g·L^-1浓度土壤浸提液中分别添加0、25、50、100、200 mg·L^{- 1}GSH,进行黄瓜、南瓜种子萌发试验,分别测定发芽率、主根长度、根系表面积、根尖数、α-淀粉酶活性、淀粉酶总活性和脯氨酸含量等指标。结果表明,土壤浸提液处理后,黄瓜和南瓜种子的发芽率、发芽指数、活力指数、主根长度、根尖数、根系表面积、α-淀粉酶活性、淀粉酶总活性得到显著抑制,脯氨酸含量升高。在自毒作用下,外施不同浓度GSH,可不同程度地提高种子发芽率、发芽指数、活力指数、主根长度、根系表面积、α-淀粉酶活性、淀粉酶总活性,降低脯氨酸含量。土壤浸提液能对黄瓜、南瓜种子产生自毒作用,添加外源GSH能诱导黄瓜和南瓜种子内淀粉酶活性增强,有效缓解自毒作用对黄瓜、南瓜种子萌发和幼苗生长的抑制作用,并且以100 mg·L^-1GSH缓解效果最显著。本研究结果为制定缓解嫁接黄瓜连作障碍措施提供了理论依据。

Abstract:The experiment was simulated autotoxicity stress by soil extract of grafted cucumber with continuous cropping.Using the cucumber and pumpkin seeds were soaked in solution with soil extract concentration of 100 g·L^-1,supplied with 0, 25, 50, 100 and 200 mg·L^-1 GSH, investigated the seed germination rate,radicle length,radicle surface area,radicle tips,α-amylase activity,total amylase activity and proline content,in order to investigate the Glutathione (GSH) effect of relieving autotoxicity and the physiological mechanism.The results showed that soil extract obviously restrained the germination rate,radicle length,radicle surface area,radicle tips,α-amylase activity,total amylase activity and the proline content of cucumber and pumpkin seeds.Application with different concentration of GSH markedly improved germination rate of seeds,radicle length, radicle surface area,α-amylase activity, total amylase activity,and reduced proline content. According to these results demonstrate that soil extract could lead to autotoxicity for seed germination,while the exogenous GSH increased the activity of amylase and alleviated osmotic stress due to autotoxicity.The GSH concentrations of 100 mg·L^-1was the optimum concentration for alleviating grafted cucumber autotoxins. The experiment provided a theoretical basis for the development of measures to alleviate the continuous cropping obstacle of grafted cucumber.

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