Abstract:In order to clear the potential value of K305ms,thirty combinations were produced by crossing ten tests with three kinds of genotypes: maize male sterile plants (msms) and fertile plants (Msms) from K305ms system and wild-type (cv. K305, MsMs) through incomplete diallel cross mode. The main characteristics of the combining ability and heterosis were investigated.The results showed that no significant differences of agronomic characteristics were found between msms and Msms. Although the values of yield characteristics of msms were similar to that of MsMs, msms exhibited remarkable increased leaf area and ear length, and remarkable decreased tassel,kernel rate and 100-seed weight, as compared to MsMs. Comparing to MsMs, msms exhibited remarkable positive effect on ear rows and remarkable negative effect on four characteristics of general combining ability (GCA) including bald tip length, suggesting that msms was an excellent parent for improving ear rows and shorting bare tip length of cross combinations. The fluctuated effects of genotypes on yield per plant of SCA in hybrid combination suggested that there was no relative correlation between genotypes of female sterility and yield of SCA. The over-standard heterosis of the yield per plant among most cross combinations was unremarkable, exhibited notable interaction effects with test sites, indicating that the multi-ecological comparisons were required to test,suggesting that K305ms system had prospects in further researches and utilization. This study could lay the foundation of maize male sterility breeding and seed production with K305ms in the future.