Abstract:CCCH zinc finger proteins are widely distributed in eukaryotic organisms, and they play important roles in plant growth, development and stress responses. To learn about sequence features and expression characters of broccoli CCCH transcription factor gene, a CCCH gene designated BoCCCH1, was isolated from broccoli (Brassica oleracea var. italica), and expression patterns of BoCCCH1 in different organs as well as leaves invaded by Hyaloperonospora parasiticawere performed by means of RT-PCR. Sequence analysis results indicated that the full length genome of BoCCCH1 was 1 568 bp with a sole intron of 599 bp. The two exons were 627 and 342 bp in length encode 322 amino acids, the molecular weight and isoelectric point were 35296.42 Da and 8.47, respectively. Phylogenetic analysis results indicated that BoCCCH1 and its homogeneous sequences were divided into 6 groups, and it was grouped with sequences from other Cruciferae plants. Expression analysis results revealed thatBoCCCH1 expressed in leaves, stalks, young siliques, flower buds and flower, with the highest expression levels in stalks and siliques, however, no expression was detected in roots. The expression were increased in leaves when infected by H. parasitica, and the highest expression levels were identified on 24 h and 36 h, indicating BoCCCH1 with resistance responses against H. parasitica. Isolation and expression analysis of BoCCCH1 will provide evidences for further studies on gene functions during downy mildew resistance responses.