Abstract:In order to study the influence diversity of different cultivation measures on grain yield, the experiment was conducted in the Institute of Crop Science, Chinese Academy of Agriculture Sciences from the year of 2013 to 2014. Strong gluten wheat (Jimai 20) and middle gluten wheat (Zhongmai 8) were used as material in a complete split-plot experimental design of three factors. The main-plot was nitrogen application amount with three levels (150 kg N·hm-2, 210 kg N·hm-2, and 270 kg N·hm-2). The sub-plot was row spacing with two levels of 12cm and 20cm), and the sub treatment was varieties (Jimai 20 and Zhongmai 8). The main results indicated that the grain yield of Jmai 20 and Zhongmai 8 was significantly increased at the level of 270 kg N·hm-2. Under the condition of 270 kg N·hm-2 or 210 kg N·hm-2, the treatment of spaced 20 cm was more beneficial to the formation of high yield. Under the condition of the treatment 270 kg N·hm-2 and spaced 20 cm, the physiological index such as SPAD readings and photosynthetic rate of flag leaves maintained higher value, which was conducive to accumulation of photosynthate and formation of highyielding. In the present experiment,grain yield of Jimai 20 and Zhongmai 8 both got the highest level in the treatment 270 kg N·hm-2 and spaced 20 cm. The experiment was obtained the best combination between nitrogen amounts and row spacing of high-yield. The findings that provided a certain guidance significance for winter wheat under high-yield cultivation.