Abstract:To identify the distribution of entomogenous fungi in the maize rhizosphere, the entomogenous fungi which were collected from counties of Guoyang, Xiaoxian, and Mengcheng in the northern of Anhui Province were isolated from maize rhizosphere soil by selective medium. The isolates were classified and identified by morphological characters and molecular method (rDNA ITS sequence analysis). The results revealed that thirty-nine entomogenous fungi identified as Beauveria bassiana were isolated from maize rhizosphere soil, and there were significant differences in the colony growth rate and sporulation quantity of different strains. It has important significance in bio-controlling underground pests and soil-borne diseases in maize.