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期 刊 ：核农学报 2010年 24卷 4期 页码：772-776

关键词：羊骨；木瓜蛋白酶；胰蛋白酶；酶解物；免疫调节；

Keywords:sheep bone, papain, trypsin, enzymatic hydrolysate, immunomodulation,

摘 要 ：摘要：以羊骨粉为底物，用木瓜蛋白酶和胰蛋白酶制备复合酶解物，用不同的剂量给小鼠灌胃（ig.），通过碳粒廓清试验、噻唑蓝法以及定量溶血法检测不同剂量酶解物对小鼠非特异性免疫、细胞免疫以及体液免疫功能的影响。结果表明：低剂量（05g/kg·d, ig，14d）可显著提高小鼠吞噬细胞清除碳颗粒的能力（P<005），中剂量（1g/kg·d, ig，14d）和高剂量（3g/kg·d, ig，14d）组的吞噬能力与对照组无显著差异（P>005）。但这2个剂量却使小鼠脾脏B淋巴细胞合成的抗体量显著高于对照组（P<005），且中剂量组显著高于高剂量组（P<005）。001mg/ml复合酶解物显著促进了T淋巴细胞对ConA的反应性（P<005）。因此适当剂量的羊骨酶解物，能提高小鼠的非特异性免疫、体液免疫和细胞免疫能力。

Abstract:Abstract：Sheep bone powder was sequentially hydrolyzed by papain and trypsin. Carbon expurgation test, MTT（thiazoyl blue tetrazolium bromide） colorimetric method and quantitative hemolysis of SRBC test were used to evaluate the effect of the prepared hydrolysate on mice innate immunity, cellmediated immunity (CMI) and humoral immunity, respectively. The results indicated that the hydrolysate at low dose（05g/kg·d, ig，14d）significantly enhanced the carbon clearance ability of macrophagocytes  compared with the control group(P<005)，but hydroly sate at medium dose (1g/kg·d, ig，14d)and high dose（3g/kg·d, ig，14d）were observed no significant differences compared with contol. These medium and high dose remarkably increased the amount of antibodyproducing cells in the spleen, and the low dosage would not. 001mg/ml hydrolysate significantly improved the ConA induced proliferation of spleen T cells (P<005). It is concluded that appropriate dose of sheep bone hydrolysate could improve the nonspecific immunity, cellmediated immunity and humoral immunity.

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