Abstract:Cell suspension culture of bamboo is difficult. The suspension cell line of Cephalostachyum fuchsianum was established for the first time in this study. Seed embryos of Cephalostachyum fuchsianum were used to induce calli. The calli were dedifferentiated from the embyos on the medium of 3/4MS+500mg/L proline+500mg/L glutamine+300mg/L tryptone+3.0mg/L 2,4-D+1.5mg/L KT +30g/L sucrose +5g/L agar. A fine suspension cell line could be established by inoculating the calli in the medium of MS+500mg/L proline+500mg/L glutamine+300mg/L tryptone+1.5mg/L 2,4-D+30g/L sucrose after 3 times shaking subcultures. The subculture cycle was 9d. The calli could be acquired from suspension cell line after the cells being cultured for 25d in the solid medium, and the appropriate inoculation density was 20g/L.