Abstract:The essential oil was obtained by steam distillation, followed by solvent extraction from the aerial parts of Adenostemme lavenia (L.) D. Ktze.. The yield of the essential oil reached 0.990% after steam distillation for 10 hours. The constituents of the obtained volatile oil were analyzed by GC-MS, and their relative contents were determined by peak area normalization. Thirty-six peaks were detected from the volatile oil, of which 35 chemical compounds were identified, accounting for 99.56％ of the volatile oil. The main components were α-cubebene (32.62%), caryophyllene (24.9%) and γ-elemen (5.53%). Some other monoterpenes and sesquiterpenes, such as α-caryophyllene (3.97%), α-chamigrene (3.57%), bicyclo [4,3,0]-7-methylene-2,4,4-trimethyl-2-vinyl nonane (3.41%), γ-terpinen (3.07%), d-limonene (2.57%), α-pinene (2.49%) and 2-Carene (2.28%) were also identified.