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作 者 ：游向荣,许鸿川,梁文裕,陈清西,郑少泉,陈伟

期 刊 ：热带亚热带植物学报 2010年 18卷 3期 页码：288~292

关键词：龙眼；成花逆转；差异蛋白；ANS；克隆；表达；

Keywords:Longan, Floral reversion, Differential protein, ANS, Clone, Expression,

摘 要 ：运用蛋白质组学比较龙眼(Dimocarpus longan Lour.)正常成花和成花逆转花芽的蛋白质组变化，结果表明，ANS蛋白(anthocyanidin synthase)在龙眼成花逆转花芽中下调表达。应用 RACE方法克隆ANS蛋白的全长cDNA，长度为1477 bp，包括1个1071 bp的开放阅读框，编码357 bp的氨基酸序列，GenBank的登录号为FJ479616(GI：218202927)。将ANS在大肠杆菌中表达，获得1个约46 kD的外源蛋白。这说明ANS蛋白在龙眼成花逆转过程中起作用。

Abstract:The changes in proteome of normal flower and floral reversion buds in longan (Dimocarpus longan Lour.) were studied by using Proteomics method. The results showed that ANS (anthocyanidin synthase) protein was found down-regulated expression in floral reversion buds. ANS cDNA was obtained (GenBank access number: FJ479616) by using RACE method, its full length was 1477 bp, with a 1071 bp open read frame. ANS gene was expressed in E. coli, and a 46 kD heterologous protein was obtained. It suggested that ANS protein play a role in floral reversion in longan.

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