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作 者 ：孙丽敏；裴雁曦；刘志强*

期 刊 ：植物研究 2016年 36卷 1期 页码：97-104

关键词：H2S；ABA；WRKY转录因子；

Keywords:H2S, ABA, WRKYtranscriptionfactors,

摘 要 ：气体信号分子硫化氢（H₂S）和植物激素脱落酸（ABA）都可以调节植物的种子萌发、根生长、气孔运动、逆境响应等生理过程，但两者之间的关系了解非常有限。转录因子WRKY18、WRKY40和WRKY60参与拟南芥对ABA信号的响应。本文以拟南芥野生型（WT），lcd（L型半胱氨酸脱巯基酶编码基因缺失突变体），wrky18wrky40wrky60（WRKY18、WRKY40和WRKY60编码基因缺失三突变体）为实验材料，研究在幼苗根生长和气孔运动过程中，H₂S与ABA信号及WRKY之间的相互作用。实验结果表明：ABA处理可诱导WT的LCD基因表达量和内源H₂S含量升高；外源H₂S处理WT，WRKY40表达量下降，WRKY60表达量升高；lcd中WRKY18和WRKY40表达量升高，WRKY60表达量下降。幼苗根生长和气孔运动实验表明：与WT相比，lcd根生长和气孔运动受ABA影响作用较小；H₂S对wrky18wrky40wrky60根生长的促进作用明显，并促进wrky18wrky40wrky60和WT气孔关闭，但两者间无显著差异。

Abstract:Gaseous signal molecule hydrogen sulfide(H₂S) and plant hormone abscisic acid(ABA) modulate plant seed germination, root growth, stomatal movement, stress responses and other physiological processes, but the understanding of the relation between H₂S and ABA signaling is very limited. The transcription factors of WRKY18, WRKY40 and WRKY60 involve in ABA signaling in Arabidopsis. We used the Arabidopsis thaliana wild type(WT), lcd(L-cysteine desulfhydrase enzyme encoding gene konckdown mutant) and wrky18wrky40wrky60 mutant(WRKY18, WRKY40 and WRKY60 encoding gene knockout triple mutants) to study the interactions of H₂S, ABA and WRKY transcription factors in regulating seedling root growth and stomatal movement. The expression level of LCD and the content of endogenous H₂S in WT were increased by ABA treatment. The expression level of WRKY40 was decreased and WRKY60 was increased in WT by exogenous H₂S treatment. The expression level of WRKY18 and WRKY40 were increased, and WRKY60 was decreased in the lcd mutant. Compared with WT, the effect of ABA on seedling root growth and stomatal movement in lcd was weaker, the promoting effect of H₂S on root growth was more stronger in wrky18wrky40wrky60, but there were no obvious difference between WT and wrky18wrky40wrky60 on H₂S promoting the stomatal closure.

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