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作 者 ：管清杰1,2；汪振娟1；郑恒1；刘光涛3；柳参奎1

期 刊 ：植物研究 2015年 35卷 2期 页码：259-269

关键词：LSD-Like基因家族；转录因子；OsLOL2基因；表达模式分析；非生物逆境胁迫；

Keywords:LSD-Likegenefamily, transcriptionfactor, OsLOL2gene, expressionpatternanalysis, abioticstress,

摘 要 ：从水稻龙粳11（Oryza sativa L. longjing11）中克隆到了一个已知的LSD1-Like型锌指蛋白转录因子OsLOL2基因(LOC_Os12g41700)。生物信息学分析表明,OsLOL2基因ORF长度为519 bp，编码172个氨基酸残基，有2个LSD1-Like锌指结构域。本研究以基因枪转化洋葱表皮细胞定位表明在核中。实时荧光定量PCR检测到OsLOL2基因在龙粳11叶和根中的表达受80 mmol·L^-1 NaCl、30 mmol·L^-1 NaHCO₃、10 mmol·L^-1 H₂O₂胁迫诱导而转录表达显著增加。过表达OsLOL2基因酵母表现出抗盐碱性生长；在胁迫条件下，萌发率分析表明，转OsLOL2拟南芥在NaCl和H₂O₂下，过表达株系萌发率高于野生型；萌发后期抗性检测表明：在含100 mmol·L^-1 NaCl、3 mmol·L^-1 NaHCO₃和2 mmol·L^-1 H₂O₂的1/2MS平板上，过表达株系长势优于野生型，揭示了OsLOL2基因能够提高植物的抗盐碱性，与盐碱逆境引起的氧化胁迫的ROS途径相关。为OsLOL2基因参与逆境信号途径的研究提供了理论依据。

Abstract:A known LSD1-Like type zinc finger protein transcription factor OsLOL2 gene(LOC_Os12g41700) was cloned from the rice Longjing 11(Oryza sativa L. longjing11). By bioinformatics analysis, the length of the ORF of OsLOL2 gene is 519 bp, which encoded 172 amino acid residues, with 2 LSD1-Like zinc finger domain. The transformation of epidermal cells of onion by Gene gun shows OsLOL2 locates in the nucleus. By real-time quantitative PCR, the transcriptional level of OsLOL2 gene increased significantly in Longjing 11 roots and leaves induced under 30 mmol·L^-1 NaHCO₃, 80 mmol·L^-1 NaCl, and 10 mmol·L^-1 H₂O₂ stress condition. Overexpression of OsLOL2 gene in yeast showed resistance to saline growth. Under stress condition, by the germination rate analysis, the germination rate of transgenic Arabidopsis was higher than that of wild type under NaCl and H₂O₂ stress. The germination rate and resistance on germination late showed the growing of overexpression strain was better than WT on 1/2 MS plate which contained 100 mmol·L^-1 NaCl, 3 mmol·L^-1 NaHCO₃ and 2 mmol·L^-1 H₂O₂. OsLOL2 gene could improve resistance for plant salt alkaline, which was related to the ROS way of oxidative stress by salt-alkali stress. Our study can provide a theoretical basis for studying OsLOL2 gene involved in stress signal way and lay the foundation for creating of new rice with stress resistance.

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