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作 者 ：高洁1；王元忠2；黄衡宇1*

期 刊 ：植物研究 2016年 36卷 1期 页码：52-57

关键词：绿花百合；胚性愈伤组织；小鳞茎；组织培养；

Keywords:Lilium fargesii Franch, embryonic callus, bulblet, tissue culture,

摘 要 ：建立绿花百合（Lilium fargesii Franch.）胚性愈伤组织诱导体系，为保护和合理利用这一重要植物资源提供高效、稳定的再生技术途径。以绿花百合鳞片为外植体，通过正交实验研究不同激素种类及其质量浓度对绿花百合胚性愈伤组织诱导、胚状体和小鳞茎分化及植株再生的影响。结果表明：较适宜诱导胚性愈伤组织的培养基为MS+6-BA 0.5 mg·L^-1+NAA 0.5 mg·L^-1+2,4-D 0.1 mg·L^-1，出愈率达89.29%，小鳞茎发生系数亦达4.7；胚性愈伤组织增殖及小鳞茎发生培养基为MS+6-BA 1.0 mg·L^-1+2,4-D 0.1 mg·L^-1，繁殖倍数5.0/35 d；根的诱导则在1/2MS+NAA 0.2 mg·L^-1的培养基上进行，生根率达100%，幼苗移栽至排水良好的沙土中，保温保湿培养35 d后，成活率可达90%以上。本研究为保持绿花百合优良品种特性、种苗繁育提供了有效途径，也为保护其野生资源，发展人工栽培和利用胚状体进行无性育种奠定了基础。

Abstract:In order to rationally protect and utilize the important wild traditional Chinese medicine resources, we built an efficient and instability regeneration system of Lilium fargesii Franch. by embryonic callus induction. With the scales as explants, by an orthogonal experiment, we studied the effect of different kinds and contents of plant hormone on embryonic callus induction and plantlet regeneration, and established a propagation system by tissue culture. The optimal medium for inducing embryonic callus was MS+6-BA 0.5 mg·L^-1+NAA 0.5 mg·L^-1+2,4-D 0.1 mg·L^-1, the callus induction rate was 89.29%, and the generation coefficient of bulblet was 4.7. The optimal medium for regeneration of embryonic callus and inducing the bulblets was MS+6-BA 1.0 mg·L^-1+2,4-D 0.1 mg·L^-1, and its regeneration rate was 5.0/35d. The optimal rooting medium was 1/2MS+NAA 0.2 mg·L^-1, and the rooting rate was 100%. There were 90% of plantlet surviving after transplanting rooted plantlets into sand when cultured in constant temperature and humidity condition for 35 d. Our study could provide a basis on protecting the improved trait of wild resources of L.fargesii, and lay the foundation for expanding the artificial cultivation and embryoid asexual breeding.

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