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作 者 ：金建邦1,3；程龙霞1,3；施曼1,3；祝遵凌1,2,3*

期 刊 ：植物研究 2015年 35卷 5期 页码：684-691

关键词：欧洲鹅耳枥；叶片；愈伤组织；诱导；分化；

Keywords:Carpinus belulus, leaves, callus, induction, differentiation,

摘 要 ：以欧洲鹅耳枥带芽叶片作为外植体，研究不同激素种类及其配比对愈伤组织诱导、增殖及分化的影响。结果表明：0.2 mg·L^-1 2,4-D的出愈率最高为96.76%；0.5 mg·L^-1 IBA处理的出愈率为97.22%；0.5 mg·L^-1 NAA的愈伤组织诱导率最高为95.03%。而WPM+0.5 mg·L^-1 BA+0.1 mg·L^-1 NAA+0.5 mg·L^-1 IBA的出愈率为92.83%，WPM+0.5 mg·L^-1 BA+0.5 mg·L^-1 NAA+0.01 mg·L^-1 IBA的出愈率为96.44%，而且诱导出的愈伤组织质量要优于单一激素诱导出的愈伤组织，因此最适合作为欧洲鹅耳枥叶片的诱导培养基。WPM+BA 2.0 mg·L^-1+KT 2.0 mg·L^-1+NAA 0.05 mg·L^-1组合愈伤组织增殖效果最好，最适合欧洲鹅耳枥叶片愈伤组织的增殖培养。WPM+BA 0.5 mg·L^-1+NAA 0.1 mg·L^-1+2,4-D 0.1 mg·L^-1组合愈伤组织诱导分化效果最好，分化率达13.54%。

Abstract:We used the leaves of Carpinus betulus as explants to culture on WPM medium, and studied the effects of the different types and concentration of plant hormones on the induction, proliferation and differentition of callus. The rate of callus was 96.76%, highest at 0.5 mg·L^-1 2,4-D. The inducing rate of callus was 97.22% at 0.5 mg·L^-1 IBA. The inducing rate of callus was 95.03% at 0.5 mg·L^-1 NAA. The callus rate was 92.83% of WPM+0.5 mg·L^-1 BA+0.1 mg·L^-1 NAA+0.5 mg·L^-1 IBA and the callus rate was 96.44% of WPM+0.5 mg·L^-1 BA+0.5 mg·L^-1 NAA+0.01 mg·L^-1 IBA. The quality of calli preceded the calli indeced by single hormone, and two treatments were most suitable for inducing of leaves of European hornbeam. WPM+BA 2.0 mg·L^-1+KT 2.0 mg·L^-1+NAA 0.05 mg·L^-1 possessed the best proliferation effect and was appropriate for callus proliferation. WPM+BA 0.5 mg·L^-1+NAA0.1 mg·L^-1+2,4-D 0.1 mg·L^-1 had the best differentiation rate with the differentiation rate of 13.54%.

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