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作 者 ：刘英；李德文；孟庆焕；张衷华；段喜华*

期 刊 ：植物研究 2015年 35卷 5期 页码：746-750

关键词：长春花；生物碱；基因表达；

Keywords:Catharanthus roseus, alkaloids, gene expression,

摘 要 ：以长春花(Catharanthus roseus(L.)G.Don)幼苗（45 d）为材料，采用温室水培法，设定不同浓度的聚乙二醇6000(Polyethyleneglycol,PEG6000)胁迫处理，通过分析叶片脯氨酸含量和相对含水量的变化，选定35%（V/V）浓度的PEG6000模拟干旱胁迫。结果显示，干旱处理12~24 h，叶片中碱性POD活性明显增强。高效液相色谱技术分析表明，干旱处理下，叶片中文多灵和长春质碱含量逐渐升高且高于对照，然后下降；处理12~24 h，长春碱含量逐渐升高，24 h达到峰值(0.17 mg·g^-1 FW±0.003 6)。半定量RT-PCR分析表明，在胁迫处理3 h，生物碱合成途径中3个基因(Tdc、Str、Dat)表达均相对升高，相关性分析表明，PEG6000干旱胁迫下，长春花叶片中Tdc基因表达与长春质碱含量变化正相关，POD的活性与长春碱含量变化正相关(P<0.05)。

Abstract:Catharanthus roseus seedlings of 45d were grown in water-culture. The different PEG6000 concentration(15%, 20%, 25%, 30%, 35%, 40%(V/V)were set to treat seedlings in greenhouse. The effect of on the proline content and the relative water content(RWC) of leaves were determineted, 35% PEG6000 concentration was selected. On the first 12-24 h hours under 35% PEG6000 treatment, the POD activities were increased gradually compared to control. High performance liquid chromatography analysis showed, there were gradual increase and then decrease in the contents of vindoline(VIN) and catharanthine(CAT) under stress, but the content of VBL increased gradually and reached the maximum on the 24 h hour of treatment. In addition, the data from the SQ-RT-PCR determination indicated that the genes(Tdc, Str, Dat) expression level were higher than the control on the 3th hour of treatment. The further correlation analysis indicated that CAT accumulation was significantly correlated with Tdc gene expression, and VBL accumulation was significantly correlated with POD activities(P<0.05).

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