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作 者 ：王凯;吴祥云;段海侠;李娜;张丽娜

期 刊 ：植物研究 2014年 34卷 5期 页码：634-641

关键词：叶绿素；SPAD；耐阴性；树种选择；

Keywords:chlorophyll, SPAD, shade tolerance, tree choice,

摘 要 ：采用分光光度计和便携式叶绿素计测定辽西北主要绿化树种叶绿素含量与SPAD值，并对二者进行相关性分析。结果表明，辽西北主要绿化树种叶片叶绿素a/b为2.35~3.83，当欧氏距离为10时，可将树种划分为3类，即低叶绿素a/b（平均值为2.50）树种6个，中等叶绿素a/b（平均值为2.97）树种29个，和高叶绿素a/b（平均值为3.52）树种20个，3者差异显著（P<0.05）。低叶绿素a/b往往反映出植物更能利用弱光，上述结果能够一定程度上反映辽西北各树种耐阴程度的高低。各树种单位鲜重和单位面积叶绿素含量及SPAD值变化范围分别为0.73~4.61 mg·g^-1、0.21~0.99 g·m^-2和29.42~75.51；变异系数分别为43.6%、35.2%和23.0%。SPAD值与单位鲜重叶绿素b和总叶绿素含量均无显著相关关系（P>0.05），而与单位面积叶绿素a、叶绿素b和总叶绿素含量均呈极显著正相关（P<0.001），并且与叶绿素a的相关性（R=0.67）高于总叶绿素（R=0.61），高于叶绿素b（R=0.56）。辽西北主要绿化树种叶绿素含量较低，SPAD值可以反映种间单位面积叶绿素含量差异，而不能反映单位鲜重叶绿素含量，根据各树种的耐阴性可以进行合理的树种选择与配置。

Abstract:Using spectrophotometry method and portable chlorophyll meter SPAD-502, the chlorophyll content and SPAD value of main greening tree species in northwestern Liaoning Province were measured, and the relationships between chlorophyll content and SPAD value were investigated in the present study. The results showed that chlorophyll a/b ranged from 2.35 to 3.83. When the euclidean distance was 10, the greening tree species could be divided into 3 groups: 6 low, 29 medium, and 20 high chlorophyll a/b tree species. The mean was 2.50, 2.97 and 3.52, respectively, and the differences were significant(P<0.05). Low chlorophyll a/b indicated better using of weak light for plant. The above results showed the shadetolerance for tree species in northwestern Liaoning Province. The unit fresh weight, unit area chlorophyll content, and SPAD value varied in ranges of 0.73-4.61 mg·g^-1, 0.21-0.99 g·m^-2 and 29.42-75.51, respectively, with the variation coefficients of 43.6%, 35.2% and 23.0%, respectively. The SPAD value was insignificantly correlated with unit fresh weight chlorophyll b and chlorophyll a+b content(P>0.05), but was significantly positively correlated with unit area chlorophyll a, chlorophyll b, and chlorophyll a+b content(P<0.001). Furthermore, the relationship was ranked as: chlorophyll a(R=0.67)>chlorophyll a+b(R=0.61)>chlorophyll b(R=0.56). These results indicated that chlorophyll content of main greening tree species was lower in northwestern Liaoning Province. SPAD values could be used to represent the difference in interspecific unit area chlorophyll content, but could not reflect the unit weight chlorophyll content. Tree choice and allocation could be made properly according to shade tolerance.

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