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作 者 ：路祺1,2；彪林海1,2；徐蕾1,2；孟永斌1,2；祖元刚1,2*；聂思铭1,2；孟庆涣1,2

期 刊 ：植物研究 2015年 35卷 5期 页码：791-794

关键词：塔拉；纤维剩余物；漂白；酶动力学；米氏方程；

Keywords:Tara, fiber residues, bleaching, enzyme kinetics, Michaelis equation,

摘 要 ：对塔拉纤维剩余物和漂白塔拉纤维剩余物的酶动力学进行了研究，采用Lineweaver-Burk双倒数法对底物样品浓度倒数和酶促反应速度倒数作图构建米氏方程，通过计算两种底物的K_m值，即塔拉纤维剩余物K_m为12.73 mg·mL^-1，漂白塔拉纤维剩余物K_m为192 mg·mL^-1，表明塔拉纤维剩余物比漂白后的塔拉纤维剩余物更适合做纤维素酶的酶解底物；红外光谱图分析表明漂白塔拉纤维剩余物中木质素成分含量明显降低。

Abstract:We studied the enzyme kinetics of Tara fiber residues before and after bleaching. By the Lineweaver-Burk double reciprocal plot method, we used the reciprocals of sample concentration and enzymatic reaction rate to plot and construct Michaelis equation. K_m value of two substrates were 12.73 mg·mL^-1 and 192 mg·mL^-1 by calculating, respectively, and the Tara fiber residues was more suitable than the bleached Tara fiber residues. The sample of bleached Tara fiber residues had lower lignin content by FTIR.

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