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作 者 ：张玉红;曲伟娣

期 刊 ：植物研究 2008年 28卷 2期 页码：236-239

关键词：黄檗；快速繁殖；培养条件；

Keywords:Phellodendron amurense Rupr., rapid propagation, culture conditions,

摘 要 ：以黄檗带顶芽和腋芽的茎段为材料，以MS和1/2MS为基本培养基，进行黄檗组培苗的快速繁殖，以研究不同培养条件对黄檗快速繁殖的影响。结果表明：以MS+6-BA 0.8 mg·L^-1为培养基，浓度10％次氯酸钠溶液消毒黄檗茎段8 min，其组培苗生长最旺盛，成活率可达92.8%。以MS为基本培养基，蔗糖浓度20 mg·L^-1，pH值5.8对黄檗组培苗的壮苗效果最好。

Abstract:Phellodendron amurense Rupr. stem segment with axillary bud and top bud were cultured with MS and 1/2MS medium in this experiment，in order to study the effects on rapid propagation of P. amurense in different culture conditions.The results showed that MS+6-BA0.8 mg·L^-1 culture medium and taking antisepsis for stem segment with axillary’s bud with 10% javel water eight minutes were the best.The survival rate of transplanting reached to 92.8%.MS was the optimum medium type. Sucrose concentrations 20 g·L^-1 and pH5.8 were the optimal culture condition for enhance culture of P. amurense seedling.

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