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作 者 ：张腾国;王圆圆;王娟;王宁;张艳;孙万仓;陈琼琼;夏惠娟

期 刊 ：植物研究 2012年 32卷 5期 页码：578-583

关键词：陇油6号；基因克隆；BnMKK2基因；表达分析；

Keywords:Longyou 6, Gene cloning, BnMKK2 gene, expression analysis,

摘 要 ：通过实验从陇油6号油菜中克隆得到了一种新的MAPK激酶基因BnMKK2基因的cDNA，全长1 344 bp,其中包括5′非翻译区（5′UTR）111 bp，3′非翻译区（3′UTR）165 bp，开放阅读框（ORF）长1 068 bp，编码355个氨基酸，该基因编码的蛋白质分子量为39.3 kDa，理论等电点为6.8。与拟南芥AtMKK2有很高的同源性，因此命名为BnMKK2(GenBank登录号:HQ848661)。该基因实时荧光定量PCR分析表明，BnMKK2基因的表达受低温胁迫诱导。

Abstract:A new MAPKK cDNA BnMKK2 was isolated from Brassica napus Longyou 6. The cloned full-length cDNA was 1 344 bp, contained a 5′ untranslated region (5′UTR) of 111 bp, a 3′ untranslated region (3′UTR) of 165 bp, and a 1 068 bp ORF. BnMKK2 encoded a protein of 355 amino acids with a deduced molecular weight of about 39.3 kDa and with a PI of 6.8. The BnMKK2 exhibited closest homology to the Arabidopsis thaliana AtMKK2, so it was named BnMKK2(GenBank accession: HQ848661). Sequence Analysis indicated that the BnMKK2 protein exhibited closest homology to MAPKK, which is responsed to cold stress, and BnMKK2 played an important role in defending cold stress. Analysis by real-time RT-PCR indicated that the expression of BnMKK2 was increased in response to cold stress.

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