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作 者 ：高彩球;刘桂丰;王玉成;姜静;杨传平*

期 刊 ：植物研究 2010年 30卷 1期 页码：81-86

关键词：Tadir基因；基因克隆；序列分析；柽柳；实时荧光定量PCR；

Keywords:Tadir gene, gene cloning, sequence analysis, Tamarix androssowii, real-time PCR,

摘 要 ：在柽柳cDNA文库测序中获得了Tadir基因的全长cDNA序列，去除PolyA后，该基因全长724 bp。其中5′非翻译区26 bp，3′非翻译区143 bp，开放阅读框（ORF）长555 bp，编码184个氨基酸。基因编码蛋白的分子量为19.69 kD，理论等电点为6.96。疏水性分析表明，蛋白的前41个氨基酸为亲水性的。该基因的Genbank登录号为DQ462418（基因），ABE73781（蛋白）。实时荧光定量PCR分析结果表明，0.4 mol·L^-1 NaCl和NaHCO₃胁迫后该基因表达量发生变化，其可能与柽柳的耐盐性有关。

Abstract:A dirigent-like protein (DIR) full length cDNA was cloned from Tamarix androssowii cDNA library. The Tadir gene is 724 bp in length without its polyA sequence, including 26 bp of 5′untranslated region and 143 bp of 3′untranslated region. It has an open reading frame (ORF) of 555 bp, encoding 184 amino acid residues. The protein molecular weight is 19.69 kD and its isoelectric point is 6.96. Hydrophobicity analysis showed that there was a hydrophilic region between 1 to 41 residues. This gene was accepted by Genbank, the accession numbers are DQ462418 (gene) and ABE73781 (protein). Under the stress of 0.4 mol·L^-1 NaCl and NaHCO₃, expression of Tadir gene has been changed by real-time PCR. Therefore, this gene may be associated with salt resistance of T.androssowii.

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