Abstract:To explores effects of light quality on anthraquinones constituents in roots of Rumex gmelini Turcz. Herbs were treated under red, yellow, green and blue films, together with a control (CK) of no shading. An HPLC method was applied to determinate the content of anthraquinones constituents of R. gmelini at different development stages. Statistical analysis was carried out using the ANOVA test. Compared with treated and blank groups, contents of anthraquinones in roots of 2-year-old Rumex gmelini Turcz. were increased significantly under yellow film shading. The content of anthraquinones in roots of 1-year-old Rumex gmelini Turcz. were increased significantly in blank and compared groups. Conclusions is contents of anthraquinones in roots of 2-year-old Rumex gmelini Turcz. were increased significantly by yellow film shading.