Abstract:An experiment was conducted to study the inhibitory activities of aqueous extracts，soaking solution with water and extraction solution with aether from Bupleurum scorzoneraefolium seeds on seed germination of B.scorzoneraefolium，seed germination and seedling growth of Brassica chinesis and wheat. The B.scorzoneraefolium seeds were soaked with water，aether，ethanol and acetone, respectively，and their effects on reducing inhibitory substance were compared. The effect of endogenous inhibitory substance on seed germination was studied in suit. Results showed that，the endogenous inhibitory substance existed in B. scorzoneraefolium seeds，and the inhibitory activity of endogenous inhibitory substance on the growth of young roots is stronger than that on seed germination of B.chinesis. The inhibitory activities of the endogenous inhibitory substance on the chlorophyll content，fresh weight and the activity of α-amylase are higher than those on seed germination of wheat. The endogenous inhibitory substance also significantly inhibits the seed germination of B. scorzoneraefolium. The germination percentage increases by soaking the seeds with aether for 24 hours under room temperature. A stratification technique for B. scorzoneraefolium seeds is hereby obtained.