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作 者 ：李海生;陈桂珠*

期 刊 ：植物研究 2008年 28卷 2期 页码：205-210

关键词：海桑属；ISSR；分子鉴定；

Keywords:Sonneratia, ISSR, molecular identification,

摘 要 ：为准确鉴别海桑属植物，采用ISSR（inter-simple sequence repeat）分子标记技术，对6种海桑属植物(海桑、拟海桑、杯萼海桑、海南海桑、卵叶海桑及无瓣海桑)基因组DNA进行PCR扩增。建立了海桑属植物ISSR标记的标准程序和海桑属各物种的DNA指纹数据库。采用的11个引物共产生71个物种特征性标记，其中无瓣海桑23个，海南海桑16个，海桑15个，杯萼海桑6个，拟海桑6个，卵叶海桑5个。运用这些特征性标记，可迅速区分海桑属植物。研究表明ISSR技术是在分子水平上鉴定海桑属植物的一种行之有效的方法。

Abstract:The utility of inter-simple sequence repeat(ISSR) DNA polymorphism to distinguish 6 taxa (Sonneratia caseolaris, S. paracaseolaris, S.alba, S.hainanensis, S.ovata, S.apetala) in the genus Sonneratia was investigated. The ISSR standardized procedure and the fingerprints database of Sonneratia species were established. 11 ISSR primers used produced 71 species-specific markers for the six Sonneratia species, including 23 specific to S.apetala, 16 specific to S.hainanensis, 15 specific to S.caseolaris, 6 specific to S. alba, 6 specific to S.paracaseolaris and 5 specific to S.ovata. According to these special markers, the six Sonneratia species could be identified quickly and accurately. It was shown that the ISSR technique is a reliable and effective tool to differentiate the Sonneratia species.

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