Abstract:The effects of shading on the activity of antioxidant enzymes, chlorophyll content, photosynthetic physiology and chlorophyll fluorescence of Saxifrage stolonifera were study to provide a theoretical basis for large-scale cultivation of S.stolonifera. The results showed that, the contents of chlorophyll were enhanced, activity of antioxidant enzymes was the highest, light saturation point (LSP), maximum net photosynthetic rate (Amax) and the average diurnal variation was the highest without “siesta”, quantum yield of PSII (ΦPSⅡ), intrinsic photochemical efficiency of PSⅡ(Fv/Fm) and photochemical quenching coefficient (qP) were enhanced, non-photochemical quenching (qN) was minimum in 50% light transmittance condition. This indicates the optimum light condition of S.stolonifera in the region of Huaihua is the transmittance of about 50%.