Abstract:The variety used in this research was Liriodendron tulipifera ‘Aureo marginatum’. The differences in chlorophyll content, leaf anatomical structure and chloroplast ultrastructure between the green part and yellow part were analyzed. The result showed that chlorophyll contents in the yellow part were lower than those in the green part. The ratio of chlorophyll a to chlorophyll b in the yellow part was higher than that in the green part. In the green part, the cells of palisade tissue were slender and arranged densely, and there were more normal chloroplasts which were ellipse. However, in the yellow part, the structure of palisade tissue became loose, there were some abnormal chloroplasts, the grains in the chloroplast were disintegrated, and more starch grains appeared in the chloroplasts.