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作 者 ：毕泉鑫;金则新*;李钧敏;李建辉

期 刊 ：植物研究 2010年 30卷 1期 页码：120-125

关键词：枫香；遗传多样性；遗传分化；ISSR；

Keywords:Liguidambar fornosana, genetic diversity, genetic differentiation, ISSR,

摘 要 ：利用ISSR分子标记技术，对浙江省内枫香自然种群的遗传多样性进行分析。用10个引物对5个枫香种群共100个个体的样品DNA进行扩增，共测得135个位点，其中多态位点为118个，多态位点百分率（P）为87.41%，Shannon指数（I）为0.464 6，Nei指数（h）为0.312 2，表明枫香总体水平的遗传多样性较高。各种群的多态位点百分率平均为59.11%，Shannon指数平均为0.366 0，Nei指数平均为0.254 3。P、I、h均显示北山种群最高，天台山种群最低。AMOVA分子差异分析显示：85.49%的变异存在于种群内，14.51%存在于种群间，基因分化系数(G_st)为0.185 6，种群间的遗传分化程度较低。种群间的基因流(N_m)为2.194 4。5个种群间的平均遗传距离为0.119 9。利用UPGMA法对5个种群进行聚类分析，结果分为两大类群：白云山、天台山、北山和安岱后4个种群组成一大类群；大明山种群单独为另一类群。

Abstract:By using inter-simple sequence repeat (ISSR) markers technique, the genetic diversity of five populations of endangered relic shrub Liguidambar fornosana was studied in this paper. Using 10 primers, ISSR amplification was conducted in 100 individuals from 5 L.fornosana populations and 118 polymorphic loci were detected from total 135 loci. The total percentage of polymorphic loci(P) was 87.41%. The mean of Shannon’s Information index(I) and Nei’s gene diversity(h) at population level was 0.366 0 and 0.254 3. It suggested that the genetic diversity of BS population was the highest while that of TTS population was the lowest. Analysis of molecular differences showed that 85.49% was distributed among individuals within populations, while only 1451% was distributed among populations, The gene differentiation coefficient (G_st) among populations is 0.185 6. The gene flow among populations is 2.194 4. The average genetic distance of 5 populations was 0.119 9. Using unweighted pair group method arithmatic average(UPGMA), BYS population, TTS population, BS population and ADH population were clustered into one group; the DMS population was clustered into another group.

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