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作 者 ：张飞;王炜勇;张智;田丹青;刘建新;刘晓静

期 刊 ：植物研究 2012年 32卷 1期 页码：115-119

关键词：光萼荷属；SSR；正交实验设计；反应体系优化；指纹图谱；

Keywords:Aechmea, SSR, orthogonal experiment design, optimization of reaction system, fingerprint,

摘 要 ：利用正交试验设计优化并确立光萼荷属（Aechmea）植物SSR反应体系，构建部分光萼荷属植物的SSR分子指纹图谱。结果表明：光萼荷属植物的最佳SSR反应体系为10 μL总体积包括1×PCR buffer、Mg²⁺ 2.0 mmol·L^-1、dNTPs 200 μmol·L^-1、引物2.5 μmol·L^-1、模板DNA 90 ng和Taq DNA聚合酶1.5 U。利用该体系和6对SSR引物对15份光萼荷属植物进行扩增反应和电泳检测，其中M1、M3、M4等3对SSR引物扩增图谱清晰、多态性较高，均能将该15份光萼荷属植物鉴别出来，初步建立了15份光萼荷属植物的SSR分子指纹图谱，进一步证明该SSR反应体系稳定可靠，可以有效用于光萼荷属植物种质资源鉴定。

Abstract:The SSR reaction system was established by orthogonal design and the SSR fingerprints were constructed for some ornamental bromeliads in Aechmea in this study. The results showed that the optimal SSR reaction system of Aechmea was a total of 10 μL containing 1×PCR buffer, Mg²⁺ 2.0 mmol·L^-1, dNTPs 200 μmol·L^-1, primers 2.5 μmol·L^-1, template DNA 90 ng and Taq DNA polymerase 1.5 U. Three informative and polymorphic SSR primers, namely M1, M3 and M4, were screened out from 6 SSR primers based on the established SSR reaction system. Three molecular fingerprints were preliminarily constructed for 15 samples of Aechmea species by these three SSR primers, respectively. The establishment of SSR fingerprint proved the SSR reaction system was stable and reliable, and could be further used in germplasm identification of Aechmea.

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