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作 者 ：魏军亚;刘德兵;魏守兴;谢子四;陈业渊*

期 刊 ：植物研究 2009年 29卷 3期 页码：352-356

关键词：香蕉；SRAP；扩增条件优化；

Keywords:Musa spp., SRAP, Optimization,

摘 要 ：为建立并优化适于香蕉（Musa spp.）SRAP分析的扩增体系，对影响香蕉SRAP反应的dNTP、Mg²⁺、模板DNA、引物浓度和Taq酶用量等因素进行优化。确定的优化扩增体系为Mg²⁺ 2.5 mmol·L^-1，dNTP 250 μmol·L^-1，Taq酶1.0 U，引物0.5 μmol·L^-1，模板DNA 20 ng，10×PCR buffer 2.5 μL，在此条件下SRAP扩增香蕉基因组DNA条带清晰，多态性丰富。该体系在29个香蕉基因组中获得较好的扩增结果，可望在香蕉植物起源和进化研究中应用。

Abstract:In order to establish and optimize the SRAP molecular marker system in Musa spp.，the concentrations of Mg²⁺, dNTPs, Taq DNA polymerase, primers which affect the SRAP-PCR reactions were optimized. The optimum system was as follows: Mg²⁺ 2.5 mmol·mL^-1, dNTPs 250 μmol·L^-1, Taq DNA polymerase 1.0 U, primer 0.5 μmol·L^-1, template DNA 20 ng, 10×PCR buffer 2.5 μL.The total volume of reaction was 25 μL. Amplications were carriyed out on 29 banana cultivars genome using this optimum system. The results showed that the system was steady and reliable and would be helpful to study origin and evolution of Musa spp..

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