---

作 者 ：张宇;姜守刚;祖元刚*;汪琢;付玉杰

期 刊 ：植物研究 2010年 30卷 (3)期 页码：369-372

关键词：7木糖10去乙酰基紫杉醇；HPLC；提取分离；鉴定；正交实验；

Keywords:7-xylosyl-10-deacetylpaclitaxel, HPLC, separation and purification, identification, orthogonal test,

摘 要 ：在寻找东北红豆杉（Taxus cuspidata）中的抗癌的活性物质过程中，一种亲水性紫杉醇衍生物7-木糖-10-去乙酰基紫杉醇被筛选出来。目的是优化并建立红豆杉中7-木糖-10-去乙酰基紫杉醇的提取工艺及鉴定，本文以使用RP-HPLC测定的7-木糖-10-去乙酰基紫杉醇含量为指标,采用正交试验法对提取过程中乙醇浓度(A)、乙醇用量(B)、提取次数(C)、提取时间(D)4因素进行优化。结果从红豆杉中提取7-木糖-10-去乙酰基紫杉醇的最主要影响因素是乙醇浓度及固液比，高浓度、高用量有利于提取；提取时间和次数也极为重要，单因素实验结果以2 h/次和提取2次为最佳。结论N9(A₃B₃C₂D₁)、N8(A₃B₂C₁D₃)为优化选出的7-木糖-10-去乙酰基紫杉醇最佳提取工艺条件。

Abstract:In an effort to search for natural products from Taxus cuspidata with improved pharmacological features compared to paclitaxel,7-xylosyl-10-deacetylpaclitaxel has been isolated and identified by our group. The objective of the present study was to optimize and establish the extraction process of 7-xylosyl-10-deacetylpaclitaxel from T.cuspidata. RP-HPLC mothod was applied to determine the content of 7-xylosyl-10-deacetylpaclitaxel. The orthogonal test was adopted to examine the effects of the 4 factors including ethanol concentration, ethanol volume, number of extraction cycles and extraction time. Ethanol concentration and ethanol volume were essential factors influencing the extraction of 7-xylosyl-10-deacetylpaclitaxel. High concentration and amount of ethanol facilitated the extraction. Number or extraction cycles and extraction time were also important. Single-factor test indicated, 2 h and 2 cycles were the best. The optimum extractive condition are N9(A₃B₃C₂D₁) and N8(A₃B₂C₁D₃).

---