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作 者 ：张　磊;张含国*;李雪峰;姜　静;魏志刚

期 刊 ：植物研究 2008年 28卷 2期 页码：216-221

关键词：落叶松；种；无性系；ISSR；鉴别；

Keywords:larch, species, clone, ISSR, identify,

摘 要 ：采用ISSR分子标记技术对兴安、长白和日本落叶松种间以及不同无性系进行了鉴别。从49条引物中筛选出13条ISSR引物可以对落叶松种间和无性系间进行鉴别，特异条带个体的百分率为100%，该项技术为落叶松新品种以及良种的准确鉴别提供了新的途径和手段：其中5条引物在日本落叶松、兴安落叶松和长白落叶松不同位置扩增出特异谱带，作为种的鉴定的标准，有9条引物可以对落叶松种内不同无性系分别扩增出特异片段，进行无性系的鉴别。

Abstract:This study used ISSR molecular marker technology to identify Larix gmelinii, Larix olgensis, Larix kaempferi and different clones. Thirteen ISSR primers which screened from fortynine primers can identify among larch species and clones，and the rate of the samples with specific band is 100%, this technology offers new ways and means for accurate identification of new larch species and elite seeds: there are five primers by which L. kaempferi, L. olgensis and L. kaempferi were amplified particularly, specific bands were produced at different location, as a species identification standards, nine primers can be amplified specific fragment in different kinds of larch clones respectively. It can identify the clones.

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