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作 者 ：李双明;孙　蕊;骆　浩;孙　愫;王　莹;付玉杰*

期 刊 ：植物研究 2007年 27卷 4期 页码：500-503

关键词：东北红豆杉；紫杉醇；三尖杉宁碱；

Keywords:Taxus cuspidata, paclitaxel, cephalomannine, UV radiation,

摘 要 ：分别采用254、365 nm两种波长的紫外光对东北红豆杉鲜叶进行辐射，研究了波长、辐射时间以及样品处理方式对东北红豆杉鲜叶中紫杉醇及三尖杉宁碱含量变化的影响。结果表明，东北红豆杉鲜叶经匀浆处理后接受紫外辐射，两种波长的紫外光都可以使紫杉醇及三尖杉宁碱的含量增加，但不同波长对紫杉醇及三尖杉宁碱含量提高的趋势却不相同。365 nm的紫外光辐射2 h时使紫杉醇和三尖杉宁碱含量均提高到了最大值，两种物质含量分别提高了44.6%和53.0%，而254 nm的紫外光在辐射8 h时才达到最大值，两种物质含量分别提高了39.2%和24.3%。可以选取365 nm的紫外光对东北红豆杉鲜叶水匀浆体系进行辐射，快速高效地提高鲜叶内紫杉醇和三尖杉宁碱的含量。

Abstract:The influence of UV wavelength, time of radiation and the processing method of sample on the content of paclitaxel and cephalomannine in the fresh leaves of Taxus cuspidata was studied in the article, in which two wavelength of 254 nm and 365 nm were used. The results showed that two kinds of UV radiation could enhance the content of two compounds in the fresh leaves of Taxus cuspidata by radiating the water homogenate of the fresh leaves. But, the increase trend of two compounds was different under diverse UV radiation. The wavelength of 365 nm made the content of two compounds achieve the maximum in 2 hours. And the rate of increase were 44.6% for paclitaxel and 53.0% for cephalomannine. While the wavelength of 254 nm needed 8 hours. The rate of increase were 39.2% for paclitaxel and 24.3% for cephalomannine. Thus，the content of paclitaxel and cephalomannine in the fresh leaves of Taxus cuspidata can be enhanced rapidly and effectively by radiating the water homogenate of the fresh leaves under UV radiation.

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