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作 者 ：王莹;王文杰;许慧男;郑广宇;孙伟;祖元刚*

期 刊 ：植物研究 2011年 31卷 4期 页码：461-466

关键词：叶绿素含量；C4酶；叶绿素荧光参数；种间差异；叶片；树枝绿色组织；

Keywords:chlorophyll, C4 enzyme, chlorophyll fluorescence parameters, inter-species differences, chlorenchyma in leaf and branch,

摘 要 ：对3种C₃木本植物丁香（Syringa meyeri）、杨树（Populus alba）、落叶松（Larix gmelinii）叶片和树枝绿色组织相关指标分析发现：3种植物叶片Chl.a+b含量均显著高于树枝，但是树枝较叶片Chl.a/b比值差异不明显（丁香、落叶松）（p>0.05）或显著高（杨树）(p<0.05)。单位叶绿素内各酶活性显示，丁香树枝PEPC、PEPCK、PPDK、NADP-ME、NADP-MDH酶活性分别是叶片的2.4、4.1、3.2、4.5和1.9倍；杨树树枝分别是叶片的2.1、15、6.3、6.3和2.8；落叶松树枝分别是叶片的6.8、6.3、4.3、4和5.5倍，而单位鲜重内各酶活性显示出的树枝与叶片的差异均小于单位叶绿素的相应结果。种间比较发现，杨树叶片和树枝5种酶活性均显著高于丁香和落叶松。树枝绿色组织内C₄酶活性普遍高于相应叶片的事实说明，木质化绿色组织内可能存在C₄酶相关的生化过程。丁香与落叶松树枝的电子传递速率、PSⅡ效率大于叶片，杨树叶片的相应值大于树枝，相关分析表明，这种高的C₄酶活性并没有显著影响叶绿素荧光参数（p>0.05），显示出树枝绿色组织和C₃植物叶片内C₄酶对光合影响的复杂性。

Abstract:All these 3 species of clove, poplar and larch showed that the contents of Chl.a+b in leaf were significantly higher than those in branch, Chl.a/b had no significant differences in clove and larch(p>0.05), but Chl.a/b in branch of poplar was significantly lower than that in leaf(p<0.05). When expressed as unit of chlorophyll, the activities of PEPC, PEPCK, PPDK, NADP-ME, NADP-MDH in branch of clove were respectively 2.4 times, 4.1 times, 3.2 times, 4.5 times and 1.9 times of those in leaf; in branch of poplar were respectively 2.1 times, 15 times, 6.3 times, 6.3 times and 2.8 times of those in leaf, and the corresponding values for larch were respectively 6.8 times, 6.3 times, 4.3 times, 4 times and 5.5 times. However, the differences in enzymes activities between leaf and branch at fresh weight unit were much smaller comparing with so large differences in chlorophyll unit. Inter-species comparison showed that 5 enzymes in poplar leaf and branch were significantly higher than clove and larch. The fact that C₄ enzymatic activities in branch chlorenchyma were much higher than leaf shows that C₄ enzyme-related biochemical processes may exist in the woody chlorenchyma. Electronic transportation rate and PSⅡ efficiency of branch in clove and larch were greater than leaf and contrary tendency was found in poplar. Correlation analysis between photosynthetic parameters and C₄ enzymes showed that the high C₄ enzymatic activities generally had no influence on the chlorophyll fluorescence indices(p>0.05), indicating the complexity of C₄ enzymes functions on the photosynthesis of branch chlorenchyma and C₃ leaf.

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