---

作 者 ：祖元刚;王延兵;王慧梅;孙莲慧

期 刊 ：植物研究 2007年 27卷 5期 页码：593-595

关键词：黄檗；叶片；RNA；

Keywords:Phellodendron amuranse, leaves, RNA, isolation,

摘 要 ：以黄檗(Phellodendron amuranse Rupr.)叶片为材料，分别利用改进的盐酸胍法、Trizol法、CTAB法提取黄檗叶片总RNA，通过RNA产率、纯度、电泳图谱等分析，确立了1种从黄檗叶片中快速分离总RNA的方法。研究结果表明，改进盐酸胍法所提取的总RNA的A₂₆₀/A₂₈₀为1.928，28S和18S条带清晰谱图完整性好，而且具有产率高、时间短、成本低的特点，所提取的总RNA适用于mRNA分离、cDNA文库的建立、Northern杂交等分析。

Abstract:A method of rapid isolation of total RNA from leaves of Phellodendron amuranse Rupr. was developed. RNA was extracted by guanidine hydrochloride, Trizol regent, and CTAB, respectively with analysis the rate of yield, purity and electrophoretograms. The A₂₆₀/A₂₈₀ of RNA extracted by guanidine hydrochloride was 1.928 with two clear bands of 28S and 18S and good integrity. The feather of RNA by this method was with less cost, fast, high purity and quantity, which can meet the demands of mRNA isolation, cDNA library construction and Northern blotting.

---