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作 者 ：李雪梅;陆国芳;王振英;彭永康*

期 刊 ：植物研究 2007年 28卷 6期 页码：694-700

关键词：除草剂；叶绿体蛋白质；双向电泳；黑麦；玉米；

Keywords:herbicide, chloroplast protein, 2D-PAGE, rye, maize,

摘 要 ：研究了黑麦、玉米在经甲基胺草膦和Atrazine两种除草剂处理后叶绿素含量、叶绿体和根尖分生组织内的蛋白质组份变化，实验结果表明，0.1 mg·L^-1的Atrazine可使黑麦中的叶绿素含量下降（分别从对照的1.72±0.034 mg·g^-1 FW下降到1.62±0.05 mg·g^-1 FW、1.25±0.015 mg·g^-1 FW）。2种除草剂均可使黑麦、玉米中的蛋白质组份产生改变，如当分别用0.1 mg·L^-1的Atrazine处理时，黑麦分生组织中，有4个蛋白质斑点，斑点7、斑点18~20被诱导产生，12个蛋白质斑点，斑点6、斑点8~17、斑点21消失。玉米分生组织中，有4个蛋白质斑点，斑点5、斑点14~16被诱导产生，4个蛋白质斑点，斑点17~20消失。黑麦叶绿体中，有两个蛋白质斑点被诱导产生，13个蛋白质斑点，斑点1~13消失，但Atrazine处理不引起玉米叶绿素含量和叶绿体蛋白质组份的变化。4 mg·L^-1 APM可引起黑麦和玉米分生组织蛋白质组份变化，在黑麦中，1个蛋白质斑点被诱导产生，4个蛋白质斑点，斑点2~5消失。玉米分生组织中，15个蛋白质斑点，斑点4~5、斑点7~16、斑点21~23被诱导产生，4个蛋白质斑点，斑点1~3、斑点6消失。APM均不能引起2种作物中叶绿素含量和叶绿体蛋白质组份的变化。

Abstract:Changes in chlorophyll content, composition of chloroplast protein and root meristems protein in rye and maize treated by AmiprophoseMethyl (APM) and Atrazine were studied. Results revealed that for the concentrations of 0.1 mg·L^-1 and 1 mg·L^-1 Atrazine, chlorophyll contents of rye decreased from 1.72±0.034 mg·g-1 FW (control) to 1.62±0.05 mg·g^-1 FW and 1.25±0.015 mg·g^-1 FW, respectively. Both kinds of herbicides could induce the protein composition changes of root meristems in rye and maize, when rye and maize were treated with 0.1 mg·L^-1 Atrazine, 4 protein spots, spot 7, spot 18~20 were induced and 12 protein spots, spot 6, spot 8~17 and spot 21 were lost in root meristems of rye, 4 protein spots, spot 5, spot 14~16 were induced and 4 protein spots, spot 17~20 were lost in root meristems of maize. 2 protein spots, spot 19~20 were induced and 13 protein spots, spot 1~13 were lost in chloroplast of rye, however, Atrazine could not cause the contents of chlorophyll and chloroplast protein composition changes in maize. For the concentration of 4 mg·L^-1 APM, 1 protein spot, spot 1 was induced and 4 protein spots, spot 2~5 were lost in root meristems of rye, and 15 protein spots, spot 4~5, spot 7~16, spot 21~23 were induced and 4 protein spots, spot 1~3, spot 6 were lost in root meristems of maize. No effect of APM on the contents of chlorophyll and protein compositions of chloroplast was observed in two crops.

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