Abstract:The extracts of Potentilla discolor Bunge. were extrated by different solvents, and enriched by macroporous resins.The pharmacological effect of the different extracts from P.discolor Bunge.was tested by hyperglycemic model mice.The total flavonoids in different extracts from P.discolor Bge. were determined by UV-spectrophotometry. The result showed that the n-BuOH fractions of 50% alcohol extracts and 70% ethanolic elutes from a macroporous resins had obvious hypoglycemic action. It could notably reduce the blood glucose of hyperglycemic mice. The content of total flavonoids of the n-BuOH fractions of 50% alcohol extracts is the highest, and the contents in 70% ethanolic elutes from macroporous resins reaches 58.25%. Therefore, the n-BuOH fractions of 50% alcohol extracts and 70% ethanolic elutes from macroporous resins are the effective parts of P.discolor Bunge. for its hypoglycemic action. Flavonoids maybe the active constituents with the hypoglycemic action in P.discolor Bunge.