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作 者 ：马飞跃;段开放;王希清;秦明聪;李吉;付玉杰*

期 刊 ：植物研究 2013年 33卷 4期 页码：494-498

关键词：降香檀叶；鹰嘴豆芽素A；染料木素；离子液体微波辅助提取；BBD设计；

Keywords:Dalbergia odorifera T.Chen leaves, biochanin A, genistein, ionic liquids-based microwave-assisted extraction, Box-Behnken design,

摘 要 ：以降香黄檀可再生资源——叶子为原料，利用离子液体微波辅助提取技术对鹰嘴豆芽素A和染料木素进行提取。通过单因素实验，3因素3水平的BBD(BoxBehnken design)实验，对提取条件进行优化，确定了离子液体微波辅助提取降香檀叶中鹰嘴豆芽素A(biochanin A)和染料木素(genistein)的最佳提取工艺条件：1.00 mol·L^-1［C₄MIM］Br，提取温度为56℃，液固比18∶1，提取时间为11 min，提取功率300 W。在最佳提取条件下，鹰嘴豆芽素A和染料木素平均提取率分别可达1.598和0.939 mg·g^-1。

Abstract:In the present research, single-factor experiments and a three-variable, three-level Box-Behnken design were performed to study the ionic liquidsbased microwave-assisted extraction of biochanin A and genistein from D. odorifera T. Chen leaves. The optimum parameters were: 1.00 mol·L^-1［C₄MIM］Br, extraction temperature 56℃, liquid/solid ratio 18∶1 mL·g^-1, extraction time 11 min, irradiation power 300 W. Under the optimal conditions, the average extraction yields of biochanin A and genistein were 1.598 and 0.939 mg·g^-1, respectively.

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