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作 者 ：蔡瑾;谢树莲*;冯佳

期 刊 ：植物研究 2009年 29卷 6期 页码：763-768

关键词：钝节拟丽藻；抑菌作用；提取条件优化；

Keywords:Nitellopsis obtusa, antimicrobial activity, optimization of extraction conditions,

摘 要 ：研究了钝节拟丽藻提取液的抑菌作用，优化了提取条件。结果表明，钝节拟丽藻乙醇提取液对金黄色葡萄球菌、枯草杆菌有明显抑制作用，对大肠杆菌、变形杆菌、酿酒酵母和黄曲霉无明显抑制作用。通过抑菌圈直径方法，对影响钝节拟丽藻抑菌物质提取的条件进行了单因素试验。正交试验中的固液比和提取温度影响样品提取液对金黄色葡萄球菌和枯草杆菌的抑菌效果，作用极显著，其交互作用对金黄色葡萄球菌和枯草杆菌的抑菌效果作用显著。最佳提取条件为固液比1∶20，提取温度85℃，50%乙醇溶剂，回流提取6 h。

Abstract:The antimicrobial activity and extraction conditions of Nitellopsis obtusa was studied. The results showed that the ethanol extracts had obvious antimicrobial activities to Staphylococcus aurous, Bacillus subtilis and indistinctive activities to Proteus vulgaris, Escherichia coli, Saccharomyces cerevisiae and Aspergillus flavus. Single factor test of extraction conditions of N.obtusa was also carried of by measuring the size of anti-microbe circle diameter. Through orthogonal design and experiments, it indicated that antibacterial activity was significantly influenced by the material to solvent ratio and extraction temperature of the extracts of N.obtusa, especially to S.aurous and B.subtilis. The optimum extraction conditions of N.obtusa were obtained the material to solvent ratio 1∶20, extraction temperature 85℃, 50% ethanol solution and 6 hours extraction time.

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