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作 者 ：郑 磊;刘关君;杨传平*

期 刊 ：植物研究 2007年 27卷 2期 页码：212-217

关键词：西伯利亚蓼；rd22基因；克隆；序列分析；

Keywords:Polygonum sibricum, rd22 gene, cloning, sequence analysis,

摘 要 ：以3% NaHCO₃溶液胁迫处理48 h的西伯利亚蓼为试材，利用RACE技术，从其茎部组织克隆了脱水应答蛋白RD22的全长cDNA序列。测序后的结果分析表明，该cDNA序列全长为1 302 bp，5′非翻译区为59 bp，3′非翻译区为25 bp，开放读码框为1 218 bp，编码405个氨基酸。在氨基酸序列的C端含有一个比较保守的BURP结构域，N端含有5个重复序列THV-VGKGGV-V。信号肽检测证明该蛋白为分泌性蛋白，前21个氨基酸区域为信号肽结构。其推演的氨基酸序列与葡萄的同源性最高，达到60%。该基因已在GenBank上注册，基因序列登录号为DQ836050。

Abstract:Using RACE (rapid amplification of cDNA ends) method, the full-length cDNA of dehydration-responsive protein RD22 was cloned from Polygonum sibricum treated with 3%NaHCO₃ solution for 48. The sequence analysis shows that the rd22 gene is 1 302 bp in length, including 59 bp of 5′untranslated region, 25 bp of 3′untranslated region and 1 218 bp of open reading frame (ORF).The rd22 gene encodes a protein of 405 amino acids. The C-terminal of the RD22 protein contains a conserved BURP domain, and the N-terminal contains 5 copies of a repetitive sequence of THV-VGKGGV-V. Signal peptide prediction shows the RD22 protein is a secretory protein, and has a signal peptide structure at the first 21 amino acids region. The amino acid sequence of RD22 prutein from Polygonum sibricum has a high homology with grape of 60%. This rd22 gene has been accepted by GenBank, and the accession number of gene sequence is DQ836050.

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