Abstract:The genetic relationships among 22 species of Manglietia plants were analyzed by using inter-simple sequence repeat（ISSR) molecular-marked technique. Ten ISSR primers were selected to assess the genomes of 22 species of Manglietia plants. The result showed that a total of 595 DNA bands were amplified and 562 of which (94.45%) were polymorphic. According to the Nei-Li genetic similarity of 0.68, UPGMA method cluster analysis indicated that these 22 species were classified into 6 cluster groups, and they were classified into 12 sub-cluster groups with the genetic similarity of 0.70. The Nei-Li genetic similarities of 22 species of Manglietia ranged from 0.605 (between M.yuyuanensis and M.conifera) to 0.956 (between M.hookeri and M.wangii). The average genetic similarity is 0.698, which suggests that Manglietia have a plenty of inter-species diversity. The genetic similarity coefficient between M.hookeri and M.wangii is 0.956, and their genetic relationship is actually the closest. The result supports the classification of M.patungensis, M.yuyuanensis and M.forrestii as independent species respectively.