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作 者 ：宰学明;钦　佩*;吴国荣;王　光;闫道良

期 刊 ：植物研究 2007年 27卷 4期 页码：416-420

关键词：高温胁迫；花生幼苗；光合速率；叶绿素；叶绿体；Ca2+-ATPase；Mg2+-ATPase；Ca2+分布；

Keywords:high temperature stress, peanut seedlings, photosynthesis speed, chlorophyll, chloroplast, Ca2+-ATPase, Mg2+-ATPase, Ca2+ distribution,

摘 要 ：将水培后盆栽的花生幼苗，置于培养箱42℃高温培养，定时测定幼苗叶光合速率、叶绿素含量和叶绿体Ca²⁺-ATPase、Mg²⁺-ATPase的相对活性，并观察幼叶细胞内Ca²⁺分布的变化。试验结果表明：高温胁迫过程中，光合速率及叶绿素含量都随处理时间的延伸而下降，并呈显著正相关；叶绿体Ca²⁺-ATPase和Mg²⁺-ATPase高温胁迫过程中相对活性呈先升后降趋势，Ca²⁺-ATPase热敏性高于Mg²⁺-ATPase；高温胁迫过程中，Ca²⁺具有从胞外转运到胞质内和叶绿体中的趋势，Ca²⁺能够稳定高温胁迫下叶肉细胞膜和叶绿体的超微结构。

Abstract:Peanut seedlings potted after tank farming were cultured at 42℃ in incubator, and the photosynthesis rate, contents of chlorophyll, activities of Ca2+-ATPase, Mg²⁺-ATPase in chloroplast were measured regularly and the Ca²⁺ distribution in young leafage was observed accordingly. The results show that under high temperature stress, the photosynthesis rate and contents of chlorophyll decreased, and were positively correlated. Moreover, activities of Ca²⁺-ATPase, Mg²⁺-ATPase in chloroplast increased early and then decreased later, and the sensitivity to heat of Ca²⁺-ATPase was higher than Mg²⁺-ATPase. In addition, most of Ca²⁺ was transferred to cytosol and chloroplast across the plasmalemma, and Ca²⁺ could stabilize the structures of membrane and chloroplast under high temperature stress.

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