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作 者 ：赵习姮;李进才*

期 刊 ：植物研究 2012年 32卷 4期 页码：488-491

关键词：鹭兰；种子；乙醇；超声波；无菌繁殖；球根；

Keywords:white egret orchid, seed, ethanol, ultrasonic, sterile propagation, bulb,

摘 要 ：为建立鹭兰（Habenaria radiata）种子繁殖法，对无菌培养条件下的种子发芽和幼苗生长进行了观测，并对无菌繁殖获得的球根进行了种植试验。结果表明，鹭兰种子不进行处理难以无菌培养发芽，种子通过75%乙醇10 s+1% NaClO 10 min处理，或者40 kHz超声波2.5 min+1% NaClO 10 min处理，在1/2MS培养基的发芽率可达58%以上；在1/2MS培养基中，添加GA-3 1 mg·L^-1对叶片生长有促进作用，添加IAA 1 mg·L^-1对根生长和球根形成有促进作用，球根形成率达55%；无菌繁殖的球根贮存至春季盆栽，成苗率约72%。

Abstract:In order to establish the seed propagation method of white egret orchid, we observed the germination and seedling growth of seeds cultured under sterile condition and conducted the planting trial of bulbs from sterile propagation. The results showed that seeds without treatment are difficult to germinate in sterile culture. The germination rate of seeds after treatment of 75% ethanol for 10 s and 1% NaClO for 10 min, or treatment of 40 kHz ultrasonic for 2.5 min and 1% NaClO for 10 min is higher than 58% in 1/2MS medium; addition of 1 mg·L^-1 GA-3 in 1/2MS medium can promote leaf growth, and addition of 1 mg·L^-1 IAA can promote root growth and bulb formation, the bulb formation rate of seedlings reached 55%; sterile breeding bulbs were stored for spring potting, they have seedling formation rate of 72%.

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