Abstract:In order to explore the function of Sugarcane mosaic virus CI gene, the immuno-localization of CI protein was investigated. Partial fragment of CI gene of SCMV Beijing isolate was amplified by RT-PCR, and cloned into the expression vector pET-28a. The recombinant plasmid was transformed into E.coli BL21(DE3)pLysS. The result of SDS-PAGE analysis showed that CI gene was highly expressed by IPTG induction. The highly specific antiserum was produced when rabbit was immunized with purified fusion protein. The localization of the CI protein in maize cells of stem, vein and leaf tissues from SCMV-BJ-infected or healthy maize plants were examined by immuno-gold labeling technique,and the results indicated that this protein was localized in the phloem cell wall of SCMV-infected stems and veins.