Abstract:The HC-Pro gene of the Beijing maize isolate of Sugarcane mosaic virus(SCMV-BJ) was amplified by RT-PCR, and ligated to the expression vector pET-22b( + ). The recombinant plasmid pETHC was transformed into E.coli BL21(DE3) and then induced to express by IPTG. The results of SDS-PAGE and Western blot analysis showed that the specific fusion protein with molecular weight of 52 kDa was highly expressed by IPTG induction under the condition of 37 ℃ and was immunoreactive. Rabbit was immunized with purified fusion protein to obtain the antiserum with high specificity. The titer of the antiserum was 1 ∶ 8 192 determined by antigen coating plate-ELISA (ACP-ELISA). The antiserum is useful for identification of SCMV-BJ in plant materials and further study on HC-Pro function.