摘 要 ：从目前南方发展的优质杂交柑‘不知火’所面临的霜冻问题出发,分离、纯化四川盆地‘不知火’果实上可能存在的优势冰核活性细菌(ice-nucleation active bacteria, INA)。细菌学鉴定和室内防治试验结果表明:分离出的9株INA 细菌4株为Erwinia ananas,5株为Pseudomonas syringae pvs.,其冰核活性均受温度、菌液浓度及pH值的影响。供试的抗霜素1号、石硫合剂、代森锰锌三种化学药剂对所分离的INA细菌室内防除效果均在90%以上,其中以抗霜素1号防除效果最好。同时,在同一生态条件下的‘伏令夏橙’果实上还分离出3株对INA细菌有拮抗作用的拮抗菌。
Abstract:In order to slow down the frost damage of the superior hybrid ‘Skiranui tangerine’(C.unshiu×C.sinensis)in the southern China, 9 strains of ice nucleation active bacteria (INA) which might cause the frost damage of the fruit were isolated from the ‘Skiranui tangerine’ fruit sampled from the Pujiang County in Sichuan Basin. Through the bacteriology identification methods, 4 isolates were identified as Erwinia ananas and 5 isolates were identified as Pseudomonas syringae pvs. The activity of INA bacteria was affected by temperatures, bacteria mixture concentrations and pH values. Three experimental medicaments No.1 frost-resistant medicament, lime-sulphate medicament and macozeb tested in the indoor controlling experiments, their effect of control were above 90%,and the No.1 frost-resistant medicament showed the best controlling effects on the INA bacteria. Meanwhile, 3 isolates of antagonistic bacteria of INA bacteria were isolated from ‘Valencia’ fruit on the same ecological condition.