Abstract:In order to enhance the disease resistance of potato to bacterial diseases, peptide antibiotic (Apidaecin) from honeybee, was selected to study its feasibility in engineering potato for bacterial disease resistances. pBinPRSIHbI with another peptide antibiotic Shiva-I as control, a bivalent plant expression vector containing Apidaecin and Shiva-I genes, was introduced into potato cultivars Mira, Zhong-5-1, Favorata and Nileke via Agrobacterium tumefaciens-mediated transformation. Thirty-one Kanamycin resistant regenerated seedlings were obtained. A high efficient regeneration system has been established in potato. Results from PCR and RT-PCR analysis indicated that foreign genes were successfully integrated into the genome and transcribed in transgenic potato.