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期 刊 ：植物保护学报 2010年

关键词：显脉香茶菜；抑菌作用；活性成分；

Keywords:Rabdosia nervosa, antifungal activity, active ingredients,

摘 要 ：利用菌丝生长抑制法、柱层析、波谱分析等方法研究显脉香茶菜甲醇提取物的抑菌作用,并分离和鉴定显脉香茶菜的抑菌活性成分。结果表明,显脉香茶菜甲醇提取物对黄瓜疫霉菌和荔枝霜疫霉菌抑制作用最强且活性成分主要集中在氯仿萃取物中,包括毛栲利素、溪黄草甲素、冬凌草甲素、nodosin、表诺多星醇和延命草素等,在20μg/mL浓度下对黄瓜疫霉菌的抑制率分别为54.61%、42.73%、75.50%、59.84%、51.11%和24.13%;对荔枝霜疫霉菌的抑制率分别为49.52%、19.45%、76.81%、10.29%、2.63%和 7.09%,以冬凌草甲素抑菌作用最为显著。光学显微镜下观察发现,冬凌草甲素会导致病原菌菌丝体过度分支。

Abstract:The antifungal activity of methanol extract and active ingredients of Rabdosia nervosa were studied by using mycelial growth, column chromatography and spectral analysis. The results showed that the methanol extract presents the highest inhibitory activity against Phytophthora melonis, Peronophthora litchi and the active ingredients mainly concentrated in the chloroform extract. Six active ingredients were isolated: lasiokaurin, rabdoserrin A, oridonin, nodosin, epinodosinol and enmein. The inhibitory rates against Phytophthora melonis of six active ingredients in 20μg/mL were 54.61%, 42.73%, 75.50%, 59.84%, 51.11%, 24.13% respectively and 49.52%, 19.45%, 76.81%, 10.29%, 2.63%, 7.09% against Peronophthora litchi respectively, oridonin had the most remarkable antifungal activity. Oridonin could induce the mycelium over branch that was observed with optical microscope and its mechanism deserves further study.

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