Abstract:Four isolates of Fusarium oxysporum f.sp. fragariae were collected and isolated from strawberry fields in Shandong Province, and selected on the media containing carbendazim respectively. Resistant strains of Fusarium oxysporum f.sp. fragariae were obtained after 45-generation’s cultivation. The biological characters of the resistant strain ZY-D and sensitive strain ZY were compared, and the cross resistance of ZY-D to fungicides was studied. The results showed that after 45-generation’s selection, the resistance ratio of ZY-D to carbendazim reached 53.91 times of that of the sensitive strain. The resistant level of ZY-D after successive incubations on the fungicide free of PDA was stable. There were no differences between resistant and sensitive strains in pathogenetic ability. Meanwhile, positive cross resistance to thiophanate-methyl and 6 other fungicides was found in ZY-D. For the growth of ZY and ZY-D, the optimal temperature was 25℃, and the optimum pH value was 9. The effects of different carbon and nitrogen sources on growth and spore production of ZY and ZY-D were different.