Abstract:In order to cultivate the stable or multi-resistant transgenic plants against Tobacco etch virus (TEV) isolates via the RNA-mediated virus resistance (RMVR), the complete genomic sequence of TEV isolate from Shandong Province (TEV-SD1) was cloned by RT-PCR and 5‘-RACE. The results indicated that the full-length of TEV-SD1 genome was 9494bp, containing a 9165bp length open reading frame (ORF) which encoded a polyprotein of 3054 amino acids. Compared the sequence of TEV-SD1 with four whole genomic sequences of TEV and 15 CP gene sequences of TEV which published in the GenBank, the average similarity of nucleotide and amino acid sequence of CP gene were 96.65% and 98.31%, respectively, higher than other functional genes of TEV; the average similarity of CP gene 3‘-end sequence was 96.55%, higher than that of the 5‘-end. Phylogenetic analysis showed that the molecular variation of TEV in nature was significant related with its host. The sequence conservative analysis is of great significance to guide the selection of target sequences for RNA interference (RNAi).